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nicking reaction catalysed with the MobA89K and MobC89K proteins. The cleaved strand is then unwound by the helicase and shipped to the 89K-encoded GI-variety T4SS machinery. The VirB1-89K component is believed to work as a lytic transglycosylase, regionally opening the peptidoglycan with contributions from Another element(s) in the assembly in the